RhoA and Rac mediate endothelial cell polarization and detachment induced by T‐cadherin
- 21 January 2005
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 19 (6) , 1-23
- https://doi.org/10.1096/fj.04-2430fje
Abstract
SPECIFIC AIMST-cadherin (T-cad) is an atypical GPI-anchored member of the cadherin superfamily, which does not mediate intercellular adhesion and has been proposed to function as a signaling receptor regulating directed cell migration. During embryogenesis T-cad acts as a negative guidance cue for extending neurites. In vascular tissue T-cad is up-regulated under conditions (atherosclerosis, restenosis, and tumor angiogenesis) associated with abnormal cell migration, growth, and phenotypic modulation. In cultured human umbilical vein endothelial cells (HUVEC), homophilic T-cad ligation induces cell polarization to a migratory phenotype, decreases cell adhesion to the substratum, and facilitates cell motility. Signaling mechanisms mediating T-cad effects have never been investigated. The present study aimed to elucidate a potential role for RhoA/Rho-kinase (ROCK) and Rac pathways in T-cad-induced endothelial cell polarization.PRINCIPAL FINDINGS1. RhoA activation is necessary but not sufficient for T-cad-in...Keywords
Funding Information
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (31-58826.99, 31-105406.1)
- Novartis Stiftung für Medizinisch-Biologische Forschung
This publication has 56 references indexed in Scilit:
- Downregulation of N-cadherin in the neointima stimulates migration of smooth muscle cells by RhoA deactivationCardiovascular Research, 2004
- Minimal Mutation of the Cytoplasmic Tail Inhibits the Ability of E-cadherin to Activate Rac but Not Phosphatidylinositol 3-Kinase: DIRECT EVIDENCE OF A ROLE FOR CADHERIN-ACTIVATED Rac SIGNALING IN ADHESION AND CONTACT FORMATIONPublished by Elsevier ,2003
- Rac Regulates Endothelial Morphogenesis and Capillary AssemblyMolecular Biology of the Cell, 2002
- VE-Cadherin Regulates Endothelial Actin Activating Rac and Increasing Membrane Association of TiamMolecular Biology of the Cell, 2002
- Cadherin Engagement Regulates Rho family GTPasesJournal of Biological Chemistry, 2001
- Contact-Dependent Inhibition of Cortical Neurite Growth Mediated by Notch SignalingScience, 1999
- Density‐ and proliferation status‐dependent expression of T‐cadherin, a novel lipoprotein‐binding glycoprotein: a function in negative regulation of smooth muscle cell growth?FEBS Letters, 1998
- T‐cadherin and signal‐transducing molecules co‐localize in caveolin‐rich membrane domains of vascular smooth muscle cellsFEBS Letters, 1998
- Differential Targeting of T- and N-cadherin in Polarized Epithelial CellsPublished by Elsevier ,1996
- Glycosyl phosphatidylinositol--anchored T-cadherin mediates calcium-dependent, homophilic cell adhesion.The Journal of cell biology, 1992