The effect of kinetin on the incorporation of labelled orotate into various fractions of ribonucleic acid of excised radish leaf discs

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Abstract
Discs from senescing radish leaves were floated either on water or on a solution of kinetin and incubated in the dark. The kinetin-treated discs were fed with orotic acid-5-T and the control discs with orotic acid-6-C14. The two lots of discs were combined and the RNA extracted by the phenol method. The H3:C14 ratio of the RNA after fractionation on sucrose density gradient was compared with the H3:C14 ratio of RNA extracted from two lots of control discs, one fed with orotic acid-5-T and the other with orotic acid-6-C14 and then extracted together. In this way it was found that 21 hours treatment with kinetin causes a small but consistent stimulation of labelled precursor incorporation into all classes of RNA investigated.