Neuron‐Specific Phosphorylation of Alzheimer's β‐Amyloid Precursor Protein by Cyclin‐Dependent Kinase 5
- 1 September 2000
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 75 (3) , 1085-1091
- https://doi.org/10.1046/j.1471-4159.2000.0751085.x
Abstract
The mature form of Alzheimer's P-amyloid precursor protein (APP) is phosphorylated specifically at Thr(668) in neurons. In mature neurons, phosphorylated APP is detected in neurites, with dephosphorylated APP being found mostly in the cell body. In vitro, active cyclin-dependent kinase 5 (Cdk5) phosphorylated the cytoplasmic domain of APP at Thr(668). Treatment of mature neurons with an antisense oligonucleotide to Cdk5 suppressed Cdk5 expression and significantly diminished the level of phosphorylated APP, The expression of APP was unaffected in antisense-treated neurons. These results indicate that in neurons APP is phosphorylated by Cdk5, and that this may play a role in its localization.Keywords
This publication has 30 references indexed in Scilit:
- Identification of the 23 kDa subunit of tau protein kinase II as a putative activator of cdk5 in bovine brainPublished by Wiley ,2001
- Phosphorylation of Alzheimer β-Amyloid Precursor-like ProteinsBiochemistry, 1997
- Neuronal Cdc2-like Kinase (Nclk) Binds and Phosphorylates the Retinoblastoma ProteinJournal of Biological Chemistry, 1997
- Ectodomain Phosphorylation of β-Amyloid Precursor Protein at Two Distinct Cellular LocationsJournal of Biological Chemistry, 1997
- Lithium inhibits glycogen synthase kinase-3 activity and mimics Wingless signalling in intact cellsCurrent Biology, 1996
- In Vitro Phosphorylation of the Cytoplasmic Domain of the Amyloid Precursor Protein by Glycogen Synthase Kinase‐3βJournal of Neurochemistry, 1996
- Neuronal cdc2-like kinaseTrends in Biochemical Sciences, 1995
- A brain-specific activator of cyclin-dependent kinase 5Nature, 1994
- p35 is a neural-specific regulatory subunit of cyclin-dependent kinase 5Nature, 1994
- The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptorNature, 1987