Diagnostic changes in plasma creatine kinase isoforms early after the onset of acute myocardial infarction.
- 1 July 1986
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation
- Vol. 74 (1) , 105-109
- https://doi.org/10.1161/01.cir.74.1.105
Abstract
Conventional plasma isoenzyme and enzyme values usually are normal during the first few hours of acute myocardial infarction. Thus definitive diagnosis may be delayed. We have shown recently that infarction in dogs can be detected within 1 hr after coronary occlusion by analysis of relative activities of MM creatine kinase (CK) isoforms in plasma. Isoforms of MM CK evolve through posttranslational modifications in plasma of the form released from tissue (MMA) to MMB and MMC. In this study we quantified changes in isoform profiles in the first available plasma samples from patients with evolving myocardial infarction, from patients with angina, and from normal subjects. In the 26 control subjects, the ratio of MMA to MMC was 1.09 +/- 0.4 (SE) (range 0.31 to 3.1; upper limit of normal [defined as the mean plus 2 SD] 2.5). In the seven control patients with coronary artery disease, the ratio of MMA to MMC was 1.3 +/- 0.3 with a range of 0.5 to 2.5. In contrast, among the 28 patients with acute myocardial infarction, the ratio of MMA to MMC in the first available plasma sample averaged 14.6 +/- 4.5 (p less than .01 compared with both control groups). First available samples were obtained 3.9 +/- 0.4 hr after the onset of pain. In 24 of 28 patients (86%) the ratio of MMA to MMC was greater than 2.5.(ABSTRACT TRUNCATED AT 250 WORDS)This publication has 16 references indexed in Scilit:
- Sensitive quantification of isoforms of canine MM creatine kinase with an immunoblot procedure suitable for large numbers of samplesAnalytical Biochemistry, 1985
- Creatine kinase MM isoenzyme subforms in myocardium, cardiac lymph and blood after coronary artery occlusion in dogsCardiovascular Research, 1984
- Studies on the sub-banding of creatine kinase MM and the “CK conversion factor”Clinical Biochemistry, 1981
- Acute myocardial infarction and MB creatine phosphokinase. Relationship between onset of symptoms of infarction and appearance and disappearance of enzymeArchives of internal medicine (1960), 1980
- Further heterogeneity demonstrated for serum creatine kinase isoenzyme MM.Clinical Chemistry, 1980
- Post-synthetic changes in creatine kinase isozymes (EC 2.7.3.2)Clinica Chimica Acta; International Journal of Clinical Chemistry, 1978
- Isoelectric focusing and hybridisation experiments on creatine kinase (EC 2.7.3.2)Clinica Chimica Acta; International Journal of Clinical Chemistry, 1977
- Isoenzymes of Creatine Phosphokinase and Diagnosis of Myocardial InfarctionAnnals of Internal Medicine, 1973
- AN IMPROVED PROCEDURE FOR SERUM CREATINE PHOSPHOKINASE DETERMINATION1967