R-plasmid transfer in soil and water

Abstract
R-plasmid transfer in Escherichia coli was investigated in nutrient broth, sterile soil, and sterile stream water. Plasmid transfer occurred in broth cultures at 30 and 22 °C, but not at 15 °C. R-plasmid transfer was not observed at 30, 22, and 15 °C in nonamended sterile soil and stream water. The addition of nutrients to sterile stream water and soil allowed plasmid transfer to occur at temperatures ranging from 15 to 30 °C. R-plasmid transfer was also observed in soil adjusted from 20 to 100% of its water-holding capacity.

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