The Pea rbcS-3A Promoter Mediates Light Responsiveness but Not Organ Specificity
- 1 April 1989
- journal article
- research article
- Published by JSTOR in Plant Cell
- Vol. 1 (4) , 471-478
- https://doi.org/10.2307/3869107
Abstract
The expression of the pea rbcS-3A gene is responsive to light and restricted to chloroplast-containing cells. Previously, we identified transcriptional elements between -410 and -50 relative to the transcription start site as being sufficient for this regulated expression. Here we investigate the role of the promoter region (-50 to +15) in rbcS-3A expression. We demonstrate an interaction between the promoter and upstream sequences located between -189 and -166, which is important for high level expression. In addition we show that, when the rbcS promoter is driven by a weak test enhancer, it can confer light-responsive but not organ-specific expression upon a bacterial reporter gene.This publication has 6 references indexed in Scilit:
- Dissection of 5′ upstream sequences for selective expression of the Nicotiana plumbaginifolia rbcS-8B geneMolecular Genetics and Genomics, 1988
- Localization and conditional redundancy of regulatory elements in rbcS-3A, a pea gene encoding the small subunit of ribulose-bisphosphate carboxylase.Proceedings of the National Academy of Sciences, 1988
- Artificial combination of two cis-regulatory elements generates a unique pattern of expression in transgenic plants.Proceedings of the National Academy of Sciences, 1987
- Sequences in the pea rbcS-3A gene have homology to constitutive mammalian enhancers but function as negative regulatory elements.Genes & Development, 1987
- Constitutive and inducible Saccharomyces cerevisiae promoters: evidence for two distinct molecular mechanisms.Molecular and Cellular Biology, 1986
- The implication of a plastid‐derived factor in the transcriptional control of nuclear genes encoding the light‐harvesting chlorophyll a/b proteinEuropean Journal of Biochemistry, 1986