The region Ser333-Arg356of the α-chain of human C4b-binding protein is involved in the binding of complement C4b
- 15 February 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 317 (3) , 228-232
- https://doi.org/10.1016/0014-5793(93)81281-4
Abstract
Human C4b‐binding protein (C4BP) functions as a cofactor to factor I in the degradation of C4b and accelerates the decay rate of the C4b2a complex. In this study we describe a monoclonal antibody directed against the α‐chain of C4BP that inhibits the binding of C4b to C4BP. In order to identify the structural domain of the a‐chain of C4BP that interacts with C4b, tryptie fragments of C4BP were generated. Amino acid sequence analysis of the fragments revealed that the residues Ser333‐Arg356 of the α‐chain of C4BP contain the epitope of this antibody, and as a consequence, that this part of the α‐chain of C4BP is likely to be involved in the interaction with C4b.Keywords
This publication has 22 references indexed in Scilit:
- Structure-function studies on human C4b-binding protein using monoclonal antibodiesEuropean Journal of Immunology, 1991
- Importance of the α3‐fragment of complement C4 for the binding with C4b‐binding proteinFEBS Letters, 1990
- Protein S binding in relation to the subunit composition of human C4b‐binding proteinFEBS Letters, 1989
- Molecular cloning of the cDNA coding for proline-rich protein (PRP): Identity of PRP as C4b-binding proteinBiochemical and Biophysical Research Communications, 1989
- Amino acid sequence studies of human C4b-binding protein: N-terminal sequence analysis and alignment of the fragments produced by limited proteolysis with chymotrypsin and the peptides produced by cyanogen bromide treatmentMolecular Immunology, 1985
- Human C4b‐binding protein, C4bpFEBS Letters, 1983
- Human C4‐binding protein: N‐terminal amino acid sequence analysis and limited proteolysis by trypsinFEBS Letters, 1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- The biochemistry of complementNature, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970