Assessment of Oversulfated Chondroitin Sulfate in Low Molecular Weight and Unfractioned Heparins Diffusion Ordered Nuclear Magnetic Resonance Spectroscopy Method

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Abstract
Heparins can be contaminated with oversulfated chondroitin sulfate, OSCS, the impurity being linked to adverse clinical events that certain lots of heparins have had on humans. Here, we propose labeling of the N-acetyl peaks in 1H NMR spectra of heparins with the parameter Dt, describing the translational diffusion coefficient available from DOSY NMR. We show how DOSY can be applied as a routine method for screening the lots of heparins for obtaining the impurity profile when using 1H NMR.