Immunocompetent cells adjacent to stainless steel and titanium miniplates and screws

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Abstract
Subepithelial soft tissue and bone obtained from the implant bed in the vicinity of stainless steel and titanium miniplates and screws were evaluated with respect to the presence of immunocompetent cells. The study included tissue specimens from 19 patients, in whom the implants (14 stainless steel and 5 titanium) had been in situ for more than 6 months. The ABC (avidin-biotin-complex) immunoperoxidase staining technique using monoclonal antibodies defining T lymphocytes (CD3+), macrophages (CD11c+) and Class II MHC (HLA-DR) was performed on EDTA demineralized, frozen bone tissue, and on fresh frozen soft tissue specimens. The results showed scattered T lymphocyte clusters, small numbers of macrophages and abundant expression of HLA-DR in the soft tissue adjacent to both stainless steel and titanium implants. There was no substantial difference in tissue reactions between implants of the two materials. The demineralized bone sections disclosed presence of immunocompetent cells in the connective tissue lining the periphery of the screw holes. Metal particles were seen in both the soft tissue and the bone specimens. We conclude that a mild tissue reaction takes place in the vicinity of miniplates and screws of stainless steel and titanium, and that the intensity of the reaction indicates that the implants are well tolerated by the host tissue.

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