Effects ofE. Coli0111.B4 Lipopolysaccharide on Spin-Labelled Murine Macrophage and Hepatocyte Membranes
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Free Radical Research Communications
- Vol. 8 (1) , 47-53
- https://doi.org/10.3109/10715768909087971
Abstract
Macrophages and hepatocytes from normal and BCG-primed mice have been spin-labelled in their membranes with 5– and 16-doxyl stearic acid. Incubation of spin-labelled cells from BCG-primed animals with lipopolysaccharide from E. coli 0111 B4 produced a detectable and transient disturbance in the cell membranes as reflected by an increase in the order parameter measured from the electron spin resonance spectra of 5-doxyl-stearate. This membrane disturbance was maximal at 3–4 hours of incubation and was only detected with cells from mice primed with BCG. Spectra obtained from the 16-doxyl-stearate-labelled cells showed no change in order parameter on incubation with lipopolysaccharide.Keywords
This publication has 10 references indexed in Scilit:
- Participation of tumor necrosis factor in the mediation of gram negative bacterial lipopolysaccharide-induced injury in rabbits.Journal of Clinical Investigation, 1988
- ENDOTOXINS AND DISEASE MECHANISMSAnnual Review of Medicine, 1987
- Leukotrienes as mediators of ischemia and shockBiochemical Pharmacology, 1986
- Interleukin-1Clinical Infectious Diseases, 1984
- Membrane perturbation of macrophages stimulated by bacterial lipopolysaccharideBiochemical and Biophysical Research Communications, 1979
- B-cell activation by lipopolysaccharide. Distinct pathways for induction of mitosis and antibody production.The Journal of Experimental Medicine, 1977
- BIOCHEMICAL CHARACTERISTICS OF ACTIVATED MACROPHAGES*Annals of the New York Academy of Sciences, 1975
- Fatty acid chain flexibility in the membranes of normal and transformed fibroblasts.Proceedings of the National Academy of Sciences, 1975
- Molecular motion in spin-labeled phospholipids and membranesJournal of the American Chemical Society, 1971
- Physics and chemistry of spin labelsQuarterly Reviews of Biophysics, 1970