Internal Motion of F-Actin in 10−6–10−3s Time Range Studied by Transient Absorption Anisotropy: Detection of Torsional Motion
- 1 June 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 93 (6) , 1705-1707
- https://doi.org/10.1093/oxfordjournals.jbchem.a134312
Abstract
By means of laser flash photolysis, the transient absorption anisotropy (TAA) of the triplet probe, 5-iodoacetamide-Eosin, labeling rabbit skeletal F-actin was measured in the 10 −6 –10 −3 s time range. The TAA curve at 20°C showed a relatively slow decay phase covering several hundred microseconds and a large residual anisotropy (∼0.1 at 2 ms). After analysis with Barkley & Zimm's formula, it was concluded that the TAA of Eosin-F-actin can be approximated by the anisotropy decay due to torsional motion of F-actin.Keywords
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