Acridine Orange Staining of Purified Polyoma Virus.
- 1 October 1961
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 108 (1) , 103-105
- https://doi.org/10.3181/00379727-108-26858
Abstract
Preparations of polyoma virus purified by CsCl isodensity centrifugation stained yellow green at pH 3.8 with 0.01% acridine orange. Pretreatment with a proteolytic enzyme was necessary before development of the stain could be inhibited by DNAase. RNAase had no effect on similar preparations. These characteristics are consistent with the identification of the nucleic acid of the mature polyoma virus particle as a double-stranded DNA.Keywords
This publication has 4 references indexed in Scilit:
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- Cytological studies of mouse embryo cells infected with polyoma virus, using acridine orange and fluorescent antibodyVirology, 1961
- The nucleic acid of polyoma virusVirology, 1960
- ISOLATION OF INFECTIOUS DEOXYRIBONUCLEIC ACID FROM SE POLYOMA-INFECTED TISSUE CULTURESProceedings of the National Academy of Sciences, 1959