Parathyroid hormone release from the perfused canine thyroid-parathyroid complex isolated in situ

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Abstract
A model for direct measurement of hormone release from the canine parathyroid gland is described. The 2 separate thyroid-parathyroid gland complexes were isolated in situ and perfused independently using a synthetic medium with a well defined concentration of Ca2+. The concentration of parathyroid hormone (PTH) in the effluent was measured by radioimmunoassay, using an antiserum to bovine PTH that cross-reacts with canine PTH. Displacement curves of dilutions of effluent samples were similar to those of bovine PTH (1-84). During infusion of 1.49 mmol/l Ca2+ the PTH release was constant for more than 4 h. Variations in Ca2+ from 1.56 to 1.15 or 2.15 mmol/l induced rapid and sustained stimulation or suppression of PTH release. The retained ability of the preparation was ascertained by recording the response to infusion of Ca free medium at the end of each experiment.