Nonlinear focusing of DNA macromolecules
- 17 July 2001
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review E
- Vol. 64 (2) , 021902
- https://doi.org/10.1103/physreve.64.021902
Abstract
The present paper reports the nonlinear electrophoretic focusing techniques developed after an original idea by Chacron and Slater [Phys. Rev. E 56, 3436 (1997)]. Focusing of DNA molecules is achieved in an alternating nonuniform electric field, created in a wedge gel with hyperbolic boundaries. The fractions separated on such a wedge retained their rectilinear shape during the electrophoresis. Experiments with gel electrophoresis confirm the possibility of a noticeable nonlinear focusing of DNA molecules.Keywords
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