DEPLETION OF CELLULAR GLUTATHIONE BY EXOGENOUS SPERMINE IN V79 CELLS - IMPLICATIONS FOR SPERMINE-INDUCED HYPERTHERMIC SENSITIZATION

Google Scholar
  • 1 January 1985
    • journal article
    • research article
    • Vol. 45  (10) , 4910-4914
Abstract
The relationship between spermine-induced thermosensitization and modulation in the cellular redox state as measured by glutathione levels was studied using Chinese hamster V79 cells. Marked cellular glutathione depletion was observed for cells treated with exogenous 1 mM spermine at 37.degree. C or 43.degree. C. Glutathione depletion and thermal sensitization by spermine were found to be cell density dependent with maximum depletion and sensitization observed at low cell densities. These findings are discussed in the context that treatment of cells with exogenous polyamines such as spermine can result in cellular oxidative stress which may in part contribute to spermine-induced thermal sensitization.