Elastohydrodynamic study of actin filaments using fluorescence microscopy
- 1 August 1997
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review E
- Vol. 56 (2) , R1330-R1333
- https://doi.org/10.1103/physreve.56.r1330
Abstract
We probed the bending of actin subject to external forcing and viscous drag. Single actin filaments were moved perpendicular to their long axis in an oscillatory way by means of an optically tweezed latex bead attached to one end of the filaments. Shapes of these polymers were observed by epifluorescence microscopy. They were found to be in agreement with predictions of semiflexible polymer theory and slender-body hydrodynamics. A persistence length of 7.4±0.2 μm could be extracted.Keywords
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This publication has 17 references indexed in Scilit:
- Flexibility of Actin Filaments Derived from Thermal FluctuationsJournal of Biological Chemistry, 1995
- Polarized and depolarized dynamic light scattering study on F-actin in solution: comparison with model calculationsMacromolecules, 1994
- Measurement of the persistence length of polymerized actin using fluorescence microscopyPhysical Review E, 1993
- Dynamic scattering from semiflexible polymersMacromolecules, 1993
- Flexural rigidity of microtubules and actin filaments measured from thermal fluctuations in shape.The Journal of cell biology, 1993
- Quasielastic light scattering study of thermal excitations of F-actin solutions and of growth kinetics of actin filamentsBiopolymers, 1992
- Chain dynamics, mesh size, and diffusive transport in networks of polymerized actin: a quasielastic light scattering and microfluorescence studyMacromolecules, 1989
- Direct observation of motion of single F-actin filaments in the presence of myosinNature, 1984
- [18] Purification of muscle actinPublished by Elsevier ,1982
- Electronmicroscopic investigation of the flexibility of f-actinBiochimica et Biophysica Acta (BBA) - Protein Structure, 1977