Isolation of an endogenous clonidine‐displacing substance from rat brain
- 21 May 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 170 (2) , 387-390
- https://doi.org/10.1016/0014-5793(84)81350-2
Abstract
An endogenous substance which specifically displaces clonidine, yohimbine and rauwolscine from rat brain α2-adrenergic receptors, has been isolated. The new compound, designed clonidine-displacing-substance (CDS), has been partially purified by ion exchange chromatography, zone electrophoresis and high performance liquid chromatography (HPLC). CDS binds specifically to α2-adrenergic receptors by competing with either α2-adrenergic agonists or α2-antagonists, but has no effect on the specific binding of [3H]prazosin to α1-adrenergic receptors in rat brain membranes. In the course of isolation, CDS was shown to be neither the endogenous neurotransmitter (−)norepinephrine (NE) nor the guanyl nucleotide GTP which lowers the specific binding of α2-agonists to the α2-adrenergic receptors.Keywords
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