A QUANTITATIVE METHOD FOR THE BIOASSAY OF PROGESTIN

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Abstract
Quantitative measurements of progesterone effects in rabbits are had by measuring ovum size and the degree of glandular proliferation of the mucosa, and combining the 2 sets of measurements gives a rather precise index of hormone activity. A mean ovum diam. of 479.8 [mu] or greater in a single test animal indicates the presence of active progestin; a value of 0.3227 or greater for the ratio of glandular area (G) to total mucosa area ([image]) and a value of 49.30 or greater for the combined index (z) are similarly significant. In a single test animal a dosage of 0.78 mgm. of progesterone can almost always be detected by ovum diam. measurement; similarily the G/M ratio permits the detection of 0.45 mgm. and the combined index a dosage of 0.38 mgm. progesterone.

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