Immunological Properties of Carbohydrate and Protein Fractions of Timothy Grass Pollens

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Abstract
The effect of hot aqueous 45% phenol on timothy grass pollen extracts has been studied. Following separation into aqueous and phenolic phases, allergenic activity was recovered in the protein material in the phenol phase after precipitation with alcohol. The largely carbohydrate fraction recovered from the aqueous phase was non-allergenic. The methods used to test for allergenicity were prick-testing in the skin of allergic subjects and measurement of release of histamine from sensitised human leucocytes following challenge in vitro. In rats injected with Bordetella pertussis, homocy to tropic antibody was induced by injection of the protein fraction, but the carbohydrate fraction showed no such response.

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