Experimental Verification of Plate-Height Abnormalities in Preparative-scale Gas Chromatography

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Abstract
Column efficiency in large scale GC was investigated by using one and two meter lengths of two inch column. The isolation of the column's influence (as opposed to the effect of sample size, nonuniform heating etc.) was achieved by extrapolating to zero sample size, bypassing the heated injection port and maintaining a closely controlled temperature. Both nitrogen and helium were used as carriers. The nature of preparative scale processes was illustrated by comparison with the efficiency of an analytical column. In addition to the general efficiency loss, plate height was found to increase with length as predicted earlier. The data were analyzed by a computer program and the C1 term and other paramaters isolated. The results were shown to agree with an independent estimate, based on particle size distribution, of the efficiency loss in large columns.

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