Effects of Cyclic Nucleotides on Lipid Biosynthesis in Mouse Mammary Gland Explants
- 1 July 1983
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 173 (3) , 306-311
- https://doi.org/10.3181/00379727-173-41647
Abstract
The effects of dibutyryl cAMP, l-methyl-3-isobutyl xanthine (MIX), cGMP, dibutyryl cGMP, and 8-bromo cGMP on the rate of lipid synthesis in mouse mammary gland explants were studied. Dibutyryl cAMP at 10–4 <M selectively inhibited the effect of prolactin on the rate of [14C]acetate incorporation into lipids. At 10-3 <M, dB-cAMP inhibited the effects of insulin, insulin plus Cortisol, and prolactin. The phosphodiesterase inhibitor, MIX, inhibited both basal and prolactin-stimulated incorporation rates in a concentration-dependent fashion. These data suggest an inhibitory role for cAMP in the regulation of lipogenesis in the mammary gland. Cyclic GMP, db-cGMP, and 8-bromo cGMP were all without effect on either basal or prolactin-stimulated incorporation rates. Therefore, it appears that cGMP, by itself, is not involved in the regulation of lipogenesis in the mammary gland.This publication has 0 references indexed in Scilit: