Localization of the Human Prostatic Secretory Protein PSP94 and its mRNA in the Epithelial Cells of the Prostate
Open Access
- 8 July 1988
- journal article
- research article
- Published by Wiley in Journal of Andrology
- Vol. 9 (4) , 253-260
- https://doi.org/10.1002/j.1939-4640.1988.tb01048.x
Abstract
The histologic distribution of a prostatic secretory protein of 94 amino acids (PSP94) and its mRNA were examined simultaneously by conventional immunohistochemistry and in situ hybridization histochemistry. The results show localization of immunoreactive PSP94 and its mRNA in the epithelial cells of the prostate gland, thus providing strong evidence of PSP94 synthesis in these cells. Preliminary analysis of PSP94 by radioimmunoassay and of its mRNA by Northern blot analysis indicates that PSP94 biosynthesis in pathologic prostatic tissues is variable. The value of PSP94 as a marker of prostate gland secretory activity is discussed.Keywords
This publication has 19 references indexed in Scilit:
- Isolation from Human Seminal Plasma of an Abundant 16‐kDa Protein Originating from the Prostate, Its Identification with a 94‐Residue Peptide Originally Described As β‐InhibinJournal of Andrology, 1987
- Molecular Cloning and Sequence of the cDNA for a 94-Amino-Acid Seminal Plasma Protein Secreted by the Human ProstateDNA, 1987
- Studies on immunocytochemical localization of inhibin-like material in human prostatic tissue: Comparison of its distribution in normal, benign and malignant prostatesBritish Journal of Cancer, 1986
- The amino acid sequence of human β-microseminoproteinBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
- Evidence for the prostatic origin of immunoreactive inhibin‐like material in human seminal plasmaInternational Journal of Andrology, 1984
- Identification of proopiomelanocortin neurones in rat hypothalamus by in situ cDNA-mRNA hybridizationNature, 1983
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- The induction of ovalbumin and conalbumin mRNA by estrogen and progesterone in chick oviduct explant culturesCell, 1978
- Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-CelluloseProceedings of the National Academy of Sciences, 1972