Adaptation of an Insect Cell Line (Grace's Antheraea Cells) to Medium Free of Insect Hemolymph
- 24 March 1967
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 155 (3769) , 1565-1566
- https://doi.org/10.1126/science.155.3769.1565
Abstract
Cultures of the insect cell line derived by Grace from Antheraea eucalypti Scott [Austrocaligula eucalypti (Scott)] were successfully adapted to medium supplemented with fetal bovine serum, whole-egg ultrafiltrate, and bovine plasma albumin instead of insect hemolymph. Cells, now in their 37th passage, have a population doubling time of 2.5 days; those of unadapted cultures, 4.2 days.Keywords
This publication has 4 references indexed in Scilit:
- Growth of Japanese encephalitis virus in Grace's continous line of moth cellsVirology, 1966
- Analysis of the amino acids and sugars in an insect cell culture medium during cell growthJournal of Insect Physiology, 1966
- Cytological Observations on Cell Strains Established in Culture From Insect Ovarian TissueAustralian Journal of Biological Sciences, 1963
- Establishment of Four Strains of Cells from Insect Tissues Grown in vitroNature, 1962