MAPPING OF HOMOTHALLIC GENES HMALPHA AND HMA, IN SACCHAROMYCES YEASTS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 84  (3) , 437-451
Abstract
Two of the 3 homothallic genes, HM.alpha. and HMa, showed direct linkage to the mating-type locus at approximately 73 and 98 stranes (57 and 65 centimorgans [cM]), respectively, whereas, the other, HO, showed no linkage to 25 standard markers distributed over 17 chromosomes including the mating-type locus. To determine whether the HM.alpha. and HMa loci located on the left or right side of the mating-type locus, equations for 3 factor analysis of 3 linked genes were derived. Tetrad data were collected and were compared with expected values by .chi.2 statistics. Calculations indicated that the HM.alpha. gene is probably located on the right arm at 95 stranes (65 cM) from the centromere and the HMa locus at approximately 90 stranes (64 cM) on the left arm of chromosome III.