Type II Ca2+/calmodulin‐dependent protein kinase binds to actin filaments in a calmodulin‐sensitive manner
- 2 November 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 208 (2) , 423-426
- https://doi.org/10.1016/0014-5793(86)81061-4
Abstract
Multifunctional type II Ca2+/calmodulin-dependent protein kinase purified from rat brain cytosol was found to bind to actin filaments in vitro. The binding was saturable, and the dissociation constant for the binding was determined to be about 4 × 10−8 M. Electron microscopic observation indicated that the kinase binds to the side of actin filaments. Calmodulin inhibited the binding of the kinase to actin filaments in a Ca2+-dependent manner. The Ca2+/calmodulin-regulated binding of the kinase to actin filaments revealed here may be important for the substrate recognition of the kinase.Keywords
This publication has 17 references indexed in Scilit:
- Phosphorylation-dependent subcellular translocation of a Ca2+/calmodulin-dependent protein kinase produces an autonomous enzyme in Aplysia neurons.The Journal of cell biology, 1985
- Phosphorylation of microtubule-associated proteins by a Ca2+/calmodulin-dependent protein kinase.The Journal of cell biology, 1984
- Purification and characterization of a calmodulin-dependent protein kinase that is highly concentrated in brain.Journal of Biological Chemistry, 1983
- Ca2+– and Calmodulin‐Dependent Phosphorylation of Microtubule‐Associated Protein 2 and t Factor, and Inhibition of Microtubule AssemblyJournal of Neurochemistry, 1983
- Purification and Cahracyterization of te Brain Calmodulin‐Dependent Protein Kinase (Kinase II), Which Is involved in the Activtion of Tryptophan 5‐MonooxygnaseEuropean Journal of Biochemistry, 1983
- Phosphorylation of microtubule-associated protein 2 by calmodulin-dependent protein kinase (Kinase II) which occurs only in the brain tissuesBiochemical and Biophysical Research Communications, 1982
- Purification and Characterization of a Ca2+‐ and Calmodulin‐Dependent Protein Kinase from Rat BrainJournal of Neurochemistry, 1982
- Actin polymerization and its regulation by proteins from nonmuscle cells.Physiological Reviews, 1982
- Actin-binding proteinsTrends in Biochemical Sciences, 1982
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970