Studies on Phyto-peroxidase

Abstract
Amino acid composition of crystalline Japanese-radish peroxidase c has been determined by an automatic amino acid analyzer. Carbohydrate composition of this enzyme has also been investigated by means of the Tillmen and the Bial reactions. The analyses indicated that the peroxidase c had the following constituents in each molecule (mol. wt. 41,500): Asp36Glu24Gly34Ala23Val27Leu 27Ileu16Ser23Thr16(Cys-)10Met3Pro16Phe14Tyr2Try2His4Lys8Arg30(-CONH2)27Hexose7Pentose2Hexosamine3Protohematin1. Since this composition was quite different from that of peroxidase a, two peroxidases could not be converted from each other, but they would be synthesized in vivo independently.