Diffusion equilibria for the isolated frog's kidney

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Abstract
In the isolated kidney of 80-90% of normal frogs (Rana temporaria) inorganic sulphate was a strongly "reabsorbed" substance, the urine concn. being only 1/3 that of the plasma or less. A small group showed sulphate concentrated like urea. The normal concn. of sulphate in the plasma of 19 frogs was 6.2 [plus or minus] 0.3 mgm. S %. Inorganic sulphate passes freely in and out of the isolated frog''s kidney immersed in a sulphate-Locke fluid. Equilibrium is practically reached in 15 min. For the active kidney the equilibrium concn. (EC) tends to a value 41% that of the external sulphate, whereas the EC of the inactive kidney (cyanide) is 58%. The EC for the sartorius muscle and liver sections similarly treated were 25 and 29% of the external sulphate. Addition of cyanide slightly reduced these EC figures, while it markedly raised the EC of the kidney. The conclusions are based on large nos. of analyses, the sulphate being estimated by the titration of benzidine precipitates using the Conway burette.

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