Flow Cytometric Enumeration of Reticulocyte in the Peripheral Blood from Canine infected with Babesia gibsoni
- 12 January 1990
- journal article
- research article
- Published by Wiley in Journal of Veterinary Medicine, Series B
- Vol. 37 (1-10) , 468-472
- https://doi.org/10.1111/j.1439-0450.1990.tb01084.x
Abstract
Summary: Peripheral blood samples from dogs infected with Babesia gibsoni were analyzed by a flow cytometer for the percentage of reticulocytes after staining with a membrane‐permeable fluorochrome, thiazole orange. Though thiazole orange has been reported to stain human reticulocytes and Plasmodium‐infected erythrocytes, number of positive cells determined by the flow cytometry did not include that of erythrocytes infected with Babesia gibsoni. Analysis of 51 samples revealed a correlation coefficient of 0.96 as compared to the conventional determination by light microscopy. Separation of reticulocytes from Babesia gibsoni‐infected erythrocytes by flow cytometry with or without the stain remained unresolved.Keywords
This publication has 4 references indexed in Scilit:
- Thiazole orange: A new dye forPlasmodium species analysisCytometry, 1987
- Thiazole orange: A new dye for reticulocyte analysisCytometry, 1986
- Babesia rodhaini, Babesia bovis, and Babesia bigemina: Analysis and sorting of red cells from infected mouse or calf blood by flow fluorimetry using 33258 HoechstExperimental Parasitology, 1979
- Occurrence of Canine Piroplasmosis in the Ikoma Mountains and Experimental Infection with This Disease Originated from These and the Rokko Mountains in JapanJournal of the Japan Veterinary Medical Association, 1969