Regulation of synthesis and activity of a mutant RNA polymerase in Escherichia coli.
- 1 December 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (12) , 5416-5420
- https://doi.org/10.1073/pnas.74.12.5416
Abstract
The expression of the genes specifying the .beta. and .beta.'' subunits of RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) was examined in an E. coli strain bearing a temperature-sensitive mutation in the .beta.'' subunit gene. A shift to 42.degree. C results in a restriction of RNA chain initiation and a cessation of RNA synthesis. A shift to 39.degree. C results in only partial restriction, allowing RNA and protein synthesis to continue. The partial restriction produces a 5- to 6-fold increase in the relative transcription rate of the .beta. and .beta.'' genes and a concomitant increase in the relative synthesis rate of the .beta. and .beta.'' proteins. The transcription rate of ribosomal protein genes was also increased somewhat. Apparently, the genes specifying the .beta. and .beta.'' subunits of RNA polymerase are regulated at the level of transcription and this regulation is related to the transcription of ribosomal protein genes. This regulation of the .beta. and .beta.'' RNA polymerase subunit genes is apparently somehow triggered by a reduction in the ability of RNA polymerase to initiate transcription on the bacterial chromosome.This publication has 14 references indexed in Scilit:
- Transcription patterns of adjacent segments on the chromosome of Escherichia coli containing genes coding for four 50 S ribosomal proteins and the β and β′ subunits of RNA polymeraseJournal of Molecular Biology, 1977
- In vivo transcription of E. coli genes coding for rRNA, ribosomal proteins and subunits of RNA polymerase: Influence of the stringent control systemMolecular Genetics and Genomics, 1977
- A temperature-sensitive rIIB mutation which affects the synthesis of bacteriophage T4 rIIB proteinVirology, 1977
- Organization of bacterial genes for ribosomal components: Studies using novel approachesCell, 1976
- Recalibrated linkage map of Escherichia coli K-12.1976
- Identification of a gene for the alpha-subunit of RNA polymerase at the str-spc region of the Escherichia coli chromosome.Proceedings of the National Academy of Sciences, 1975
- New chromosomal location for structural genes of ribosomal proteins.Proceedings of the National Academy of Sciences, 1975
- Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits.Proceedings of the National Academy of Sciences, 1975
- Temperature-sensitive RNA polymerase mutants with altered subunit synthesis and degradation.Proceedings of the National Academy of Sciences, 1975
- Stringent Control of Ribosomal Protein Gene Expression in Escherichia coliProceedings of the National Academy of Sciences, 1974