Effect of Formalin Fixation on the Activity of Five Enzymes of Rat Liver
- 1 January 1951
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 26 (1) , 19-23
- https://doi.org/10.3109/10520295109113172
Abstract
The effect of formalin fixation on the enzymatic activity of five enzymes in rat liver was studied as a function of time and temperature, β-glucuronidase was found to be most resistant to the inactivating effects of formalin. At a time when fixation was satisfactory for preparing frozen sections (24 hours), considerable enzymatic activity was present in decreasing order; β-glucuronidase, sulfatase, acid phosphatase, esterase, and alkaline phosphatase.Keywords
This publication has 13 references indexed in Scilit:
- Preparation of Naphthyl Acid Phosphates1Journal of the American Chemical Society, 1950
- THE COLORIMETRIC DETERMINATION OF LIPASE AND ESTERASE IN HUMAN SERUM 1Journal of Clinical Investigation, 1950
- The comparative distribution of esterase in the tissues of five mammals by a histochemical techniqueThe Anatomical Record, 1949
- EVIDENCE FOR THE SPECIFICITY OF ESTERASE AND LIPASE BY THE USE OF THREE CHROMOGENIC SUBSTRATESJournal of Biological Chemistry, 1949
- COLORIMETRIC DETERMINATION OF LIPASE AND ESTERASE IN DOG'S SERUMAmerican Journal of Physiology-Legacy Content, 1949
- Histopathologic TechnicSouthern Medical Journal, 1948
- Effect of Acetone and Alcohol Fixation and Paraffin Embedding on Activity of Acid and Alkaline Phosphatases in Rat TissuesScience, 1948
- STUDIES ON ACTIVATION OF PURIFIED ALKALINE PHOSPHATASE1948
- Microtechnical Demonstration of Phosphatase in Tissue Sections.Experimental Biology and Medicine, 1939