Heat shock proteins and thermotolerance; a comparison of induction kinetics
- 1 August 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in The British Journal of Radiology
- Vol. 55 (656) , 579-584
- https://doi.org/10.1259/0007-1285-55-656-579
Abstract
The hypothesis that the expression of heat shock proteins following a preliminary hyperthermic treatment is responsible for subsequent thermotolerance to a second heat treatment is examined. CHO cells were given a 12 min, 45°C pretreatment and then incubated for varying intervals at 37°C. The synthesis of certain intracellular proteins was monitored as a function of time post-incubation by using 35S-methionine incorporation as determined in SDS polyacrylamide gel electrophoresis. Cell survival was concurrently measured by challenging the cells with a second heat treatment (45°C/27 min).This publication has 27 references indexed in Scilit:
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