An optimized microtiterplate assay to detect acetylcholinesterase activity in individual Chironomus riparius Meigen

1 July 2000

journal article
research article
Published by Oxford University Press (OUP) in Environmental Toxicology and Chemistry

Vol. 19 (7) , 1749-1752
https://doi.org/10.1002/etc.5620190707

Abstract

A sensitive microtiter plate assay to detect acetylcholinesterase activity in fourth‐instar Chironomus riparius (Meigen) larvae was developed and optimized to allow the measurement of pesticide inhibition in individuals. Optimal conditions, including enzyme concentration, pH, and substrate concentration, allow the sensitive measurement of changes in activity following exposure to the organophosphate insecticide chlorpyrifos. Significant reductions in activity were observed at pesticide doses of 0.05, 0.1, 1.0, and 5 mg/L following a 48‐h exposure.

Keywords

This publication has 24 references indexed in Scilit:

Laboratory and field response of Chironomus riparius to a pyrethroid insecticide
Water Research, 1999
The Use of Biomarkers to Measure the Interactive Effects of Chemicals
Ecotoxicology and Environmental Safety, 1998
Esterase activity and allele frequency in field populations of Simulium equinum (L.) (Diptera: Simuliidae) exposed to organophosphate pollution
Environmental Toxicology and Chemistry, 1997
The effect of malathion 60 on Gammarus pulex (L.) below watercress beds
Environmental Toxicology and Chemistry, 1995
The lethal and sublethal responses of gammarus pulex to stress: Sensitivity and sources of variation in an in situ bioassay
Environmental Toxicology and Chemistry, 1991
Toxicity of four common pollutants to the freshwater macroinvertebratesChironomus riparius Meigen (Insecta:Diptera) andGammarus pulex (L.) (Crustacea:Amphipoda)
Archives of Environmental Contamination and Toxicology, 1991
Use of acetylcholinesterase activity to detect sublethal toxicity in stream invertebrates exposed to low concentrations of organophosphate insecticides
Aquatic Toxicology, 1990
Mechanisms of organophosphate and carbamate resistance in Culex quinquefasciatus from Saudi Arabia
Medical and Veterinary Entomology, 1990
Rapid microtitre plate test distinguishes insecticide resistant acetylcholinesterase genotypes in the mosquitoes Anopheles albimanus, An.nigerrimus and Culex pipiens
Medical and Veterinary Entomology, 1989
The susceptibility to selected insecticides and acetylcholinesterase activity in a laboratory colony of midge larvae,Chironomus tentans (Diptera:Chironomidae)
Bulletin of Environmental Contamination and Toxicology, 1974