HUMAN GLOMERULAR SMOOTH-MUSCLE (MESANGIAL) CELLS IN CULTURE

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 34  (2) , 150-158
Abstract
Glomerular smooth muscle cells were grown and subcultured from human glomeruli in vitro. These cells, stained with antiserum to smooth muscle actomyosin and axamined by immunofluorescence, had brightly stained intracellular fibrils similar to those seen in smooth muscle cells. Actomyosin fibrils were altered by conditions affecting actomyosin in vitro. Glomerular smooth muscle cells lacked the antihemophilic factor and blood group antigens present in endothelial cells, and are probably derived from the smooth muscle cells of the glomerular mesangium. By radio-labeled amino acid analysis they synthesize a collagen differing from that of fibroblasts, which probably differs from basement membrane collagen. Other cell types could be grown and subcultured using a different glomerular isolation technique, culture medium and method of subculture.

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