Single-Molecule Fluorescence Observed with Mercury Lamp Illumination
Open Access
- 1 November 1999
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 27 (5) , 1008-1014
- https://doi.org/10.2144/99275rr01
Abstract
We demonstrate that it is possible to observe single fluorescent molecules using a standard fluorescence microscope with mercury lamp excitation and an inexpensive cooled charge-coupled device (CCD...Keywords
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