ISOLATION OF SUPPRESSIVE SENSITIVE MUTANTS FROM KILLER AND NEUTRAL STRAINS OF SACCHAROMYCES CEREVISIAE
Open Access
- 1 August 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 74 (4) , 571-579
- https://doi.org/10.1093/genetics/74.4.571
Abstract
Dominant sensitive mutants were isolated from a killer and a neutral strain of Saccharomyces cerevisiae by treatment with nitrosomethylurethane. Genetic studies suggest that these sensitives arose by mutation of the wild-type cytoplasmic genetic determinants (k) or (n) to a mutant form (s). (s) determinants lack wild-type (k) and (n) activity but are retained in the cell and suppress the replication or activity of the wild-type determinants, converting killer and neutral cells to the sensitive phenotype. These mutants show an obvious similarity in behavior to suppressive petite mutants of yeast.Keywords
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