Evidence for communication between nerve growth factor and protein tyrosine phosphorylation
- 1 October 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 271 (1-2) , 119-122
- https://doi.org/10.1016/0014-5793(90)80386-w
Abstract
Nerve growth factor (NGF) stimulation of PC12 cells activated two myelin basic protein (MBP) kinase activities > 10‐fold within 5 min, which were resolved by chromatography on Mono Q. Each enzyme phosphorylated MBP on threonine and was inactivated by incubation with either CD45, a protein tyrosine phosphatase, or protein phosphatase 2A (PP2A), a serine/threonine phosphatase. The effects of CD45 and PP2A were prevented by vanadate and okadaic acid, respectively. Activation of the MBP‐kinases provides a mechanism for communication between NGF and intracellular protein tyrosine phosphorylation.Keywords
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