ASPARAGINASE OF MYCOBACTERIA

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Abstract
Washed cells of strains H37Ra and H37Rv of Mvcobacterium tuberculosis var. hominis, strain BCG of M. tuberculosis var. bovis Mvcobacterium sp. strain 607, and M. smegmatis form ammonia from asparagine without O2 uptake and also under strictly anaerobic conditions. There is no evolution of CO2. Filter paper chromatographic analysis of the contents of the reaction vessel showed that aspartic acid is the only other product of the reaction. The enzyme, asparaginase, was extracted from cells of M. smegmatis. This enzyme is active at pH 6-9 and differs from asparaginase I in that it is not inactivated at 50[degree] C for 10 minutes and differs from asparaginase II since added pyruvate does not enhance its activity.

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