Enhancement of RNA synthesis in isolated pea nuclei by gibberellic acid.
- 1 January 1968
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 59 (1) , 269-276
- https://doi.org/10.1073/pnas.59.1.269
Abstract
Nuclei isolated from shoots of light-grown dwarf peas in the presence of gibberellic acid [GA] (10-8 [image]) show a higher rate of DNA-dependent RNA synthesis than the untreated nuclei. RNA synthesized by GA-treated nuclei has a different nearest-neighbor frequency and also has a higher average molecular weight than the RNA synthesized by untreated nuclei. Methylated albumin kieselguhr column chromatography showed that GA preferentially enhances the synthesis of the RNA associated with the DNA peak and the tenaciously bound RNA. Gibberellic acid can thus modify the RNA synthesized by isolated nuclei.This publication has 19 references indexed in Scilit:
- NUCLEIC ACID BIOSYNTHESIS IN SEED GERMINATION: INFLUENCES OF AUXIN AND GROWTH-REGULATING SUBSTANCESAnnals of the New York Academy of Sciences, 1967
- Some properties of mammalian DNA-like RNA isolated by chromatography on methylated bovine serum albumin-kieselguhr columnsJournal of Molecular Biology, 1966
- Gibberellic acid-controlled metabolism of RNA in aleurone cells of barleyBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
- Stimulation of Synthesis of Ribonucleic Acid in Sub-apical Sections of Avena Coleoptile by Indolyl-3-acetic AcidNature, 1965
- The mechanism of action of plant growth substances: The role of nuclear RNA in growth substance actionBiochimica et Biophysica Acta (BBA) - General Subjects, 1965
- An improved procedure for measuring the distribution of P32O4− among the nucleotides of ribonucleic acidAnalytical Biochemistry, 1964
- Relative Activity of the GibberellinsNature, 1962
- THE PHYSICAL STATE OF NEWLY SYNTHESIZED RNAProceedings of the National Academy of Sciences, 1961
- A fractionating column for analysis of nucleic acidsAnalytical Biochemistry, 1960
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956