Differentiation between human and chimpanzee in bloodstains by enzyme-linked immunosorbent assay (ELISA) using antihuman serum
- 1 October 1986
- journal article
- research article
- Published by Springer Nature in International journal of legal medicine
- Vol. 97 (2) , 99-103
- https://doi.org/10.1007/bf00204442
Abstract
An enzyme-linked immunosorbent assay (ELISA) for species identification of human bloodstains using two commercially available antisera against human serum is described. Human bloodstains were to be distinguished from those of chimpanzees and other animals using raw antisera, and the differentiation between human and chimpanzee became more evident when those antisera were absorbed with a small amount of chimpanzee plasma. Human bloodstains could clearly be identified by the present method even after 4 weeks of aging at room temperature. Ein enzymgekoppelter Immunabsorptions-Test (ELISA) für die Speziesidentifizierung menschlicher Blutspuren unter Verwendung zweier kommerzieller Anti-Seren gegen menschliches Serum wird beschrieben. Menschliche Blutspuren waren unterscheidbar von solchen des Schimpansen und solchen anderer Tiere unter Verwendung von Rohseren, und die Differenzierung zwischen Mensch und Schimpanse wurde eindeutiger, wenn diese Anti-Seren mit einer kleinen Menge von Schimpansenplasma absorbiert wurden. Menschliche Blutspuren konnten durch die gezeigte Methode klar identifiziert werden, dies sogar nach vierwöchiger Lagerung bei Raumtemperatur.Keywords
This publication has 5 references indexed in Scilit:
- [Identification of human hemoglobin by enzyme-linked immunosorbent assay (ELISA) using hemoglobin-coated polystyrene beads (II). Forensic immunological studies on human specificity, report-XVII].1984
- [Identification of human hemoglobin by enzyme-linked immunosorbent assay (ELISA) using hemoglobin-coated polystyrene beads--forensic immunological studies on human specificity. Report-XVI].1984
- Species Identification of Blood and Saliva Stains by Enzyme-Linked Immunoassay (ELISA) Using Monoclonal AntibodyJournal of Forensic Sciences, 1984
- Identification of human bloodstains by enzyme-linked immunosorbent assay (ELISA).1983
- The antigenicity of sera of man and animals in relation to the preparation of specific precipitating antiseraEpidemiology and Infection, 1952