Chromosomal mechanism for the induction of reversion in transformed cells

Publisher Website
Google Scholar
Abstract
The changes in chromosome constitution that occur during induction of reversion in transformed cells have been studied. The culture of transformed cells at low cell density without a feeder layer, which induces reversion, also induced a change in chromosome number from diploid to aneuploid. The percentage of revertants formed under these conditions was related to the percentage of aneuploid cells. Most of the aneuploid cells had a subtetraploid chromosome number. The results indicate that the formation of aneuploids is due to the formation from binucleate cells of polyploid cells which later lose chromosomes, and suggest that conditions that stabilize the reverted state produce the right type of stable aneuploid cells required for reversion.