A novel homologous recombination system to study 92 kDa type IV collagenase transcription demonstrates that the NFκB motif drives the transition from a repressed to an activated state of gene expression
- 8 January 2004
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 18 (3) , 540-541
- https://doi.org/10.1096/fj.03-0960fje
Abstract
SPECIFIC AIMSThe 92 kDa type IV collagenase (MMP-9) plays a major role in physiological and pathological events such as trophoblast implantation in pregnancy, bone development, and tumor progression. Regulation of MMP-9 protein levels has been largely ascribed to transcriptional activation of the gene. Considering its role in physiology and pathology, there is a need for an increased understanding of how this gene is transcriptionally controlled. In the present study, we report on the development and characterization of a novel system (which overcomes several limitations associated with studies using extrachromosomal plasmids) in which a single copy of a wild-type or mutated MMP-9 promoter fragment(s) fused to a luciferase reporter was inserted at a Flp recombinase target site (FRT) in the HT1080 cell genome.PRINCIPAL FINDINGSTo direct MMP-9 promoter-reporter constructs to a single genomic locus, we first generated constructs bearing a MMP-9 regulatory sequence-driven luciferase cDNA and a Flp FRT site. T...Keywords
Funding Information
- Dutch Dairymen Board (R01 CA89002, R01 DE 10845, P50‐CA097007)
This publication has 28 references indexed in Scilit:
- Histone H3 phosphorylation by IKK-α is critical for cytokine-induced gene expressionNature, 2003
- A nucleosomal function for IκB kinase-α in NF-κB-dependent gene expressionNature, 2003
- Ets-2 and Components of Mammalian SWI/SNF Form a Repressor Complex That Negatively Regulates the BRCA1PromoterPublished by Elsevier ,2003
- Repression of 92-kDa Type IV Collagenase Expression by MTA1 Is Mediated through Direct Interactions with the Promoter via a Mechanism, Which Is Both Dependent on and Independent of Histone DeacetylationPublished by Elsevier ,2003
- Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysisGenes & Development, 2002
- KiSS-1 Represses 92-kDa Type IV Collagenase Expression by Down-regulating NF-κB Binding to the Promoter as a Consequence of IκBα-induced Block of p65/p50 Nuclear TranslocationJournal of Biological Chemistry, 2001
- Airway Epithelial Cell Migration Dynamics: MMP-9 Role in Cell–Extracellular Matrix RemodelingThe Journal of cell biology, 1999
- Transcriptional activation of the matrix metalloproteinase-9 gene in an H-ras and v-myc transformed rat embryo cell lineOncogene, 1997
- Keratinocyte Growth Factor Stimulation of Gelatinase (Matrix Metalloproteinase-9) and Plasminogen Activator in Histiotypic Epithelial Cell CultureJournal of Investigative Dermatology, 1995
- On the structure and chromosome location of the 72- and 92-kDa human type IV collagenase genesGenomics, 1991