Therapeutic dendritic-cell vaccine for simian AIDS
- 23 December 2002
- journal article
- research article
- Published by Springer Nature in Nature Medicine
- Vol. 9 (1) , 27-32
- https://doi.org/10.1038/nm806
Abstract
An effective immune response against human immunodeficiency virus or simian immunodeficiency virus (SIV) is critical in achieving control of viral replication. Here, we show in SIV-infected rhesus monkeys that an effective and durable SIV-specific cellular and humoral immunity is elicited by a vaccination with chemically inactivated SIV-pulsed dendritic cells. After three immunizations made at two-week intervals, the animals exhibited a 50-fold decrease of SIV DNA and a 1,000-fold decrease of SIV RNA in peripheral blood. Such reduced viral load levels were maintained over the remaining 34 weeks of the study. Molecular and cellular analyses of axillary and inguinal node lymphocytes of vaccinated monkeys revealed a correlation between decreased SIV DNA and RNA levels and increased SIV-specific T-cell responses. Neutralizing antibody responses were augmented and remained elevated. Inactivated whole virus-pulsed dendritic cell vaccines are promising means to control diseases caused by immuno- deficiency viruses.Keywords
This publication has 39 references indexed in Scilit:
- Persistent numbers of tetramer+ CD8+ T cells, but loss of interferon-γ+ HIV-specific T cells during progression to AIDSBlood, 2002
- Visualizing priming of virus-specific CD8+ T cells by infected dendritic cells in vivoNature Immunology, 2002
- Eventual AIDS vaccine failure in a rhesus monkey by viral escape from cytotoxic T lymphocytesNature, 2002
- Decreased Interferon-α Production in HIV-Infected Patients Correlates with Numerical and Functional Deficiencies in Circulating Type 2 Dendritic Cell PrecursorsClinical Immunology, 2001
- In Vitro Human Immunodeficiency Virus Eradication by Autologous CD8+T Cells Expanded with Inactivated-Virus-Pulsed Dendritic CellsJournal of Virology, 2001
- Dendritic cells generated from blood monocytes of HIV-1 patients are not infected and act as competent antigen presenting cells eliciting potent T-cell responsesImmunology Letters, 1999
- Short Communication Low CD83, but Normal MHC Class II and Costimulatory Molecule Expression, on Spleen Dendritic Cells from HIV+PatientsAIDS Research and Human Retroviruses, 1998
- Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNANature Biotechnology, 1998
- Broad cross-neutralizing activity in serum is associated with slow progression and low risk of transmission in primate lentivirus infectionsImmunology Letters, 1996
- Viro-immunopathogenesis of HIV disease: implications for therapyImmunology Today, 1995