Xenopus Kielin: A dorsalizing factor containing multiple chordin-type repeats secreted from the embryonic midline
- 25 April 2000
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 97 (10) , 5291-5296
- https://doi.org/10.1073/pnas.090020497
Abstract
The midline tissues are important inductive centers of early vertebrate embryos. By signal peptide selection screening, we isolated a secreted factor, Kielin, which contains multiple cys-rich repeats similar to those in chordin (Chd). Expression of Kielin starts at midgastrula stages in the notochord and is detected in the floor plate of neurula embryos. Kielin is induced in mesoderm and in ectoderm by nodal-related genes. Chd is sufficient to activate Kielin expression in mesoderm whereas Shh or HNF-3β in addition to Chd is required for induction in ectoderm. Kielin has a distinct biological activity from that of Chd . Injection of Kielin mRNA causes dorsalization of ventral marginal zone explants and expansion of MyoD expression in neurula embryos. Unlike Chd , Kielin does not efficiently induce neural differentiation of animal cap ectoderm, suggesting that the activity of Kielin is not simply caused by BMP4 blockade. Kielin is a signaling molecule that mediates inductive activities of the embryonic midline.Keywords
This publication has 51 references indexed in Scilit:
- Zebrafish Nodal-Related Genes Are Implicated in Axial Patterning and Establishing Left–Right AsymmetryDevelopmental Biology, 1998
- The dorsalizing and neural inducing gene follistatin is an antagonist of BMP-4Mechanisms of Development, 1997
- Ectodermal Patterning in Vertebrate EmbryosDevelopmental Biology, 1997
- Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene functionNature, 1996
- Induction of motor neurons by Sonic hedgehog is independent of floor plate differentiationCurrent Biology, 1995
- The winged-helix transcription factor HNF-3β is required for notochord development in the mouse embryoCell, 1994
- HNF-3β is essential for node and notochord formation in mouse developmentCell, 1994
- Follistatin, an antagonist of activin, is expressed in the Spemann organizer and displays direct neuralizing activityCell, 1994
- Neural Induction by the Secreted Polypeptide NogginScience, 1993
- Von Willebrand factor shares a distinctive cysteine-rich domain with thrombospondin and procollagenBiochemical and Biophysical Research Communications, 1987