Alpha-subunit of human chorionic gonadotropin in fluid of molar vesicles.

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Abstract
Sera and fluid in molar vesicles from 4 patients with hydatidiform mole were analyzed for hCG [human chorionic gonadotropin] and its subunits. These samples were initially chromatographed through a standardized Sephadex G-100 upward column. Starting materials and each fraction on gel filtration were radioimmunoassayed in homologous hCG, hCG-.alpha. and hCG-.beta. assays. The specimens obtained from 4 patients contained hCG and little hCG-.beta.. Immunoreactive hCG-.alpha. was barely detectable in sera but was clearly demonstrated in vesicle fluid from molar patients. This immunoreactive hCG-.alpha. was combined with [125I]hCG-.beta.. The combined protein, [125I]hCG and [125I]hCG-.beta. recombined with urinary hCG-.alpha. were concentrated in the superovulated rat ovary in vivo. [125I]hCG-.alpha. and [125I]hCG-.beta. were not taken up by the ovaries. The biosynthesis of hCG and the possible biological role of hCG-.alpha. are discussed.