Constitutive Expression of Bacteriophage P2 Early Genes Resulting from a Tandem Duplication

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Abstract
A tandem-duplication mutant of bacteriophage P2 was isolated. Physically, its particles are characterized by a higher buoyant density and lower heat stability than the wild type, both consequences of increased DNA content. Genetically, the mutant is easily recognized by its insensitivity to control by the immunity-specific repressor. The duplication covers part of gene B, necessary for phage DNA replication. To explain the immunity-insensitivity of the duplication it is proposed that the promoter, but not the operator site, in the early gene operon is duplicated in this mutant. By crosses with a gene-B mutant, a recombinant carrying a heterozygous duplication was isolated.