Mononuclear phagocytes from human bone marrow progenitor cells; morphology, surface phenotype, and functional properties of resting and activated cells

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Abstract
SUMMARY: After 3–4 weeks culture of human bone marrow cells in medium supplemented with IL-3, macrophagc- (M-CSF), and granulocyte-macrophage colony-stimulating factor (GM-CSF), the firmly adherent cells exhibited the morphologic features of mononuclear phagocytes and were strongly esterase-positive. Flow cylometric analysis revealed a rather homogeneous cell population with marked autofluorescence; the large majority of the cells expressed CD14. CD11a,b, and c, Fc receptors for IgG, FcγRI, II, and III, and HLA class II molecules. Interfcron-gamma (IFN-γ). bacteria, and bacterial products modulated expression of some of the surface markers, induced and/ or enhanced respiratory burst, phagoeytic activity, secretion of tumour necrosis factor, and lumouricidal activity; in contrast, these cells were not able to generate reactive nitrogen intermediates.

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