Effects of inhibitors of DNA and protein syntheses on mitogenic factor production by PPD-stimulated guinea pig lymphocytes.

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Abstract
The effects of inhibitors of DNA synthesis, mitosis and protein synthesis on mitogenic factor production by lymph node cells from guinea pigs sensitized to tuberculin was studied. All inhibitors were added to the medium at the beginning of culture. Cytosine arabinoside, vinblastine and mitomycin-C markedly reduced the incorporation of 3H-thymidine into DNA, but had no effect on the production of mitogenic factor. Mitomycin-C at higher concentrations induced the partial reduction of mitogenic factor production. Puromycin and cycloheximide, inhibitors of protein synthesis, markedly suppressed the production of mitogenic factor. Mitogenic factor production apparently requires protein synthesis, but is independent of DNA synthesis.

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