A Simplified Method for the Preparation of Transcriptionally Active Liver Nuclear Extracts
- 1 December 1990
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA and Cell Biology
- Vol. 9 (10) , 777-781
- https://doi.org/10.1089/dna.1990.9.777
Abstract
We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.Keywords
This publication has 23 references indexed in Scilit:
- The liver-specific transcription factor LF-B1 contains a highly diverged homeobox DNA binding domainCell, 1989
- The pituitary-specific transcription factor GHF-1 is a homeobox-containing proteinCell, 1988
- A liver-specific factor essential for albumin transcription differs between differentiated and dedifferentiated rat hepatoma cells.Genes & Development, 1988
- Human CCAAT-binding proteins have heterologous subunitsCell, 1988
- Factors involved in control of tissue-specific expression of albumin geneCell, 1987
- A pituitary-specific trans-acting factor can stimulate transcription from the growth hormone promoter in extracts of nonexpressing cellsCell, 1987
- Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factorCell, 1987
- Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nucleiNucleic Acids Research, 1983
- [36] Eukaryotic gene transcription with purified componentsPublished by Elsevier ,1983
- Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresisNucleic Acids Research, 1981