Effect of the bile acid taurolithocholate on cell calcium in saponin‐treated rat hepatocytes
- 25 January 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 227 (2) , 161-166
- https://doi.org/10.1016/0014-5793(88)80889-5
Abstract
Neomycin was used to assess the involvement of Ins (1,4,5)P3 in the Ca2+ release from the endoplasmic reticulum induced by the bile acid taurolithocholate. In saponin‐permeabilized rat hepatocytes, neomycin via its ability to bind Ins (1,4,5)P3 abolished the release of Ca2+ induced by added Ins (1,4,5)P3. In contrast, it did not alter the Ca2+ release initiated by the bile acid. In intact cells, neomycin had no effect on the [Ca2+]i rises promoted by taurolithocholate and vasopressin. It is suggested that the effect of taurolithocholate in liver is not mediated by Ins (1,4,5)P3 but results from a primary action on endoplasmic reticulum.Keywords
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