Thermostable Inhibition of Bacterial Hyaluronidases by the Serum of Normal Human Beings

Publisher Website
Google Scholar
Abstract
The inhibiting power of serum from 50 normal humans against hyaluronidases from filtrates of cultures of type 3 pneumococcus, Hemolytic Staphylococcus aureus, Clos-tridium perfringens, and beta-hemolytic Streptococcus (group A), and from bovine testicular extract, was detd. by the mucoprotein clot prevention method immediately after removal of serum from the blood, and again after the serum had been heated at 56[degree]C for 30 min. The % of sera which inhibited before heating over the percentage which inhibited after heating is as follows for the various hyaluronidases: pneumococcus, 94/90; Staphylococcus, 47/39; Clostridium, 32/20; Streptococcus, 24/14; and testicular, 96/5. Activity of most of the thermally inactivated sera was restored by addition of complement (0.5 ml. of a 1 :30 dilution of guinea pig serum) which alone did not inhibit test strength of hyaluronidases.